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Dear Uhn-Soo, depending on the number of crystals you have, you can collect some frames on several crystals and merge the data afterward or even better, you can make a composite dataset by shooting several parts of a single crystal, sliding along an axis and by taking care of rotating your crystal the number of degrees needed between each collection in order to be able to merge your data. This method generally works to avoid averaging "fresh" and "burnt" crystal parts. Hope this helps, Virgile Quoting Uhnsoo Cho <[EMAIL PROTECTED]>: > Dear bulletin members, > > Sorry for non-CCP4 related questions. > Recently, I've got crystals which have a radiation damage problem. > It also has a weak diffraction because of the big unit cell dimension (about > 300A in one direction), so I have to overexpose crystals even with the > synchrotron beam. > The problem is that during the data collection, the resolution decaded > gradually from 3 to 6A after certain number of frames (about 30 or 40 > frames). > Do you have any experiences or any suggestions that I can evade this > radiation damage? > Any suggestions will be grateful. > Thank you. > > Best regards, > > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > Uhn-Soo,Cho > Graduate student in Biological Structure Department > University of Washington. > HSB G514 > 1959 NE Pacific St. > Seattle, WA 98195-7420 > Box. 357420 > Fax #206-543-1524 > Tel # 206-221-2435 > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > -- ***There are 10 kinds of people: those who can count in binary and the others*** ------------------------------------------------- This mail sent through IMP: http://horde.org/imp/
