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Hi, in structure 1XTF (Botulinum toxin A light chain) the c-terminal His-tag folds back into the active site of the enzyme. The carboxy-terminal His is coordinated by Zn atom of the catalytic center. cheers, christian -- Christian Stegmann Max-Planck Institute for Biophysical Chemistry Dept. Cellular Biochemistry / X-ray Crystallography Am Fassberg 11 37077 Goettingen, Germany Phone: +49-551-201-1068 Fax: +49-551-201-1197 On 8/1/06, Radisky, Evette S. Ph.D. <[EMAIL PROTECTED]> wrote:
*** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** In structures of His-tagged subtilisin cocrystallized with chymotrypsin inhibitor 2, we saw two completely different well-ordered conformations for the His-tag in two different crystal forms (1LW6 and 1TM1). In both cases, there was no "peptide binding site"; the His-tag was held in place primarily through crystal contacts. One would presume that it is disordered in solution. Cheers, Evette Evette S. Radisky, Ph.D. Assistant Professor and Associate Consultant II Mayo Clinic Cancer Center Griffin Cancer Research Building, Rm 310 4500 San Pablo Road Jacksonville, FL 32224 (904) 953-6372 (office) (904) 953-2857 (lab) -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of David Waterman Sent: Tuesday, August 01, 2006 12:49 PM To: Bret Church Cc: [email protected] Subject: Re: [ccp4bb]: Poly-Histidine Tag Structures *** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** That is interesting. Are these all crystal structures? Does anyone know of an example where a His-tag is intrinsically ordered in a crystal structure, and not just because, for example, it is being held in place by a peptide binding site? Cheers David Bret Church wrote: > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Hi Dan, > > As at 2 January 2006 there were 236 structures with at least one chain > containing 5 or more consecutive Histidine residues. Atom records were > used in order to extract out the sequences of the polypeptides. > > Gook luck. > > Erdahl Teber > Church Lab > Faculty of Pharmacy > University of Sydney > > > > > > At 12:44 PM 14/07/2006, you wrote: > >> *** For details on how to be removed from this list visit the *** >> *** CCP4 home page http://www.ccp4.ac.uk *** >> >> >> Hello: >> >> I was wondering if anyone knows how many structures there are in pdb >> with poly his-tag coordinates deposited? >> >> Thanks, >> Dan > > > _______________________________________________________ > W. Bret Church > Lecturer in Pharmaceutical Chemistry > Faculty of Pharmacy A15 > http://www.pharm.usyd.edu.au/ tel: (61-2) 9036-6569 > University of Sydney 2006 fax: (61-2) 9351-4391 > Australia email: [EMAIL PROTECTED] > http://www.sbio.pharm.usyd.edu.au > or > http://www.churchlab.asn.au > > Seminar program for Pharmacy is at > http://www.pharm.usyd.edu.au/news/index.shtml#seminar > > Drug Design Amongst the Vines, Hunter Valley, Australia 3-7 December > 2006 http://www.ddconference.com > > Sydney Protein Group Website: > http://www.mmb.usyd.edu.au/spg > > > >
