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Dear all,
Sorry this thread has started to get a bit old now, but I wanted to
reply to thank all those who shared their interesting cases of ordered
His-tags. Most of those I looked at were short tags, either at the N or
C terminal. However there was a longer, partly ordered tag, so it looks
like it is not possible to conclude that it is /always /better to remove
cleavable His-tags, though presumably in most cases it is preferable.
The resolutions of these structures are moderately good, perhaps hinting
at generally well-ordered crystals.
The suggestion that tags might bind ions well enough to use for
anomalous signal phasing is interesting. If it were possible to design a
tag that took on an ordered conformation on binding then that might be
useful, though currently the assumption that one's His-tag will be
disordered is usually correct.
summary:
2F0A, 1.80Å: ...LEHHHHHH
1HZ5, 1.80Å: MHHHHHHAM...
2A0K, 1.80Å: MAHHHHHH...
1IG3, 1.90Å: MGSSHHHHHHSSGLVPRGSH... (ordered from 10th residue)
1LW6, 1.50Å: ...QAAAQHHHHHH
1TM1, 1.70Å: ...QAAAQHHHHHH
1XTF, 2.20Å: ...PGHHHHHH
Cheers
D
David Waterman wrote:
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That is interesting. Are these all crystal structures? Does anyone
know of an example where a His-tag is intrinsically ordered in a
crystal structure, and not just because, for example, it is being held
in place by a peptide binding site?
Cheers
David
Bret Church wrote:
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Hi Dan,
As at 2 January 2006 there were 236 structures with at least one
chain containing 5 or more consecutive Histidine residues. Atom
records were used in order to extract out the sequences of the
polypeptides.
Gook luck.
Erdahl Teber
Church Lab
Faculty of Pharmacy
University of Sydney
At 12:44 PM 14/07/2006, you wrote:
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Hello:
I was wondering if anyone knows how many structures there are in pdb
with
poly his-tag coordinates deposited?
Thanks,
Dan
_______________________________________________________
W. Bret Church
Lecturer in Pharmaceutical Chemistry
Faculty of Pharmacy A15
http://www.pharm.usyd.edu.au/ tel: (61-2) 9036-6569
University of Sydney 2006 fax: (61-2) 9351-4391
Australia email: [EMAIL PROTECTED]
http://www.sbio.pharm.usyd.edu.au
or
http://www.churchlab.asn.au
Seminar program for Pharmacy is at
http://www.pharm.usyd.edu.au/news/index.shtml#seminar
Drug Design Amongst the Vines, Hunter Valley,
Australia 3-7 December 2006
http://www.ddconference.com
Sydney Protein Group Website:
http://www.mmb.usyd.edu.au/spg
