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First, what are the contact distances? If you're talking something like 2.8-3.4, then it could be an oxygen atom. Second, I assume that you're stating 1sigma in a sigma-weighted 2mFo-nFc map. The difference map peaks will probably be higher, like around 4-8 sigma. Third, put it in as a water, and refine the B's and coordinates. Is the refinement stable, and are the B's reasonable? Bernie Santarsiero On Fri, September 8, 2006 12:19 pm, Taiana Oliveira wrote: > Hi everybody, > > I´m analysing the structure of a lectin complexed with a dimanoside. I > have > a tetramer at the assymetric unit and there´s a electron density at 1 > sigma > near the ligand in all four monomers. Can I explain this density as water? > > Thanks for the attention, > > -- > Taianá Maia de Oliveira > Laboratório de Moléculas Biologicamente Ativas - Biomol-lab > Departamento de Bioquímica e Biologia Molecular > Univesidade Federal do Ceará > Campus do Pici, s/n Bloco 907 > Caixa Postal 6043 > 60.455-970, Fortaleza-Ce, Brasil > tel/fax: +55 85 33669818 >
