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By vapor diffusion. You can equilibrate over an ammonium/acetate containing
buffer system. If you desire you can do that in multiple steps. I don't know
about the speed of equilibration, but you can check that with empty drops. I
know it works in principle as I managed to mess up some crystals this way ;-)
good luck
jens
Andrew Wong wrote:
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Hi
Just a quick question.. for doign hanging drop, is there anyway that we
can decrease the pH of the drops on a (somewhat) controlled way, say over
a period of couple of weeks? Thanks
Andrew
