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You can use a volatile buffer like acetate in the reservoir to diffuse into your drop. For example, you can buffer your protein in the hanging drop with 20-100 mM Bis-Tris at pH 6.5 and use 1 mM NaAc at pH 5.0. The hanging drop will gradually approach pH 6.5 as the acetate diffuses into the hanging drop. You can affect the rate of change by altering the concentration of volatile buffer, as well as by changing the buffer to a less (or more) volatile species.
The pH change in the system I described above will probably happen over 3-6 days at RT, slower at 4C.
Regards, Song
*** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** Hi Just a quick question.. for doign hanging drop, is there anyway that we can decrease the pH of the drops on a (somewhat) controlled way, say over a period of couple of weeks? Thanks Andrew
-- Dr. Song Tan Associate Professor in Biochemistry & Molecular Biology Center for Gene Regulation Dept of Biochemistry & Molecular Biology 108 Althouse Laboratory (office & lab in 3 Althouse Laboratory) Penn State University University Park, PA 16802 email: [EMAIL PROTECTED] http://www.bmb.psu.edu/faculty/tan/lab/ phone: 814-865-3355 fax: 814-863-7024
