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Phoebe, Did you mean NaAc? NH4 does not make sense. I think pH needs to be below pK (1M NaAc does not smell as acetate at pH=7.0). Alex -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED] Sent: Monday, December 04, 2006 12:39 PM To: Andrew Wong; [email protected] Subject: Re: [ccp4bb]: Decrease pH in xtal drop? *** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** I think a low-pH well buffer with volatile components (say (NH4)acetate ) would do the job, although I don't know about speed. Maybe you could measure it with some old-fashioned pH sensitive dye in a trial drop? Phoebe At 01:08 PM 12/4/2006, Andrew Wong wrote: >*** For details on how to be removed from this list visit the *** >*** CCP4 home page http://www.ccp4.ac.uk *** > > >Hi > >Just a quick question.. for doign hanging drop, is there anyway that we >can decrease the pH of the drops on a (somewhat) controlled way, say over >a period of couple of weeks? Thanks > > >Andrew ------------------------------------------------------------------------ --------------------------------------------------- Phoebe A. Rice Assoc. Prof., Dept. of Biochemistry & Molecular Biology The University of Chicago phone 773 834 1723 fax 773 702 0439 http://bmb.bsd.uchicago.edu/index.html http://www.nasa.gov/mission_pages/cassini/multimedia/pia06064.html
