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Hi Yong, Unless you need to stick to a specific sequence alignment you made up, you may also consider using SwissModel at http://swissmodel.expasy.org//SWISS-MODEL.html. Good luck, Nadir Selon Yong Tang <[EMAIL PROTECTED]>: > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Dear all, > > I have a protein that has a homolog with known crystal structure. I > aligned them manually and very carefully to make sure that we get what > we have known right. I want to now replace the conserved residues in > the model into those of my protein of interest and hopefully also do > some sort of positional minimization so that I could have a rough idea > about the surface of my protein of interest. Would there be any > program doing this for me - that is, take the model pdb and my > alignment file and generate a model for my protein of interest? If > not, would you be kind enough to share your protocol with me if you > have done this by hand. Happy Holidays! > > Thanks! -yong > Pr. Nadir T. Mrabet Cellular & Molecular Biochemistry INSERM U-724 UHP - Nancy 1, School of Medicine 54505 Vandoeuvre-les-Nancy Cedex France Tel : +33 (0)3.83.68.32.73 Fax : +33 (0)3.83.68.32.79 E-mail : [EMAIL PROTECTED]
