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Hi Todd,
You can express them as a single polypeptide with an appropriate
linker. If you know the interacting surfaces then you can design
which goes first and which goes second with the appropriate length of
the linker. Please look at the following reference for a similar study.
Kingston et al. PNAS 2004 vol. 101, no. 22, 8301-8306
Good luck!
Anthony Addlagatta
________________________________
Anthony Addlagatta, PhD
HHMI Research Associate
Institute of Molecular Biology
University of Oregon
Eugene, OR-97403
Phone: (541) 346-5867
Fax: (541)346-5270
Web: http://darkwing.uoregon.edu/~anthony
On Dec 20, 2006, at 1:25 PM, Green, Todd wrote:
Hello all,
I am working with two proteins that have a relatively low affinity
for each other. I thought I might have more success crystallizing
this complex if I crosslinked the two proteins prior to
crystallization trials. Can someone point me in the direction of
reference material for methods or examples of proteins that have
been crosslinked and subsequently crystallized? I know this has
been asked on the ccp4bb before however I have not seen responses
to such threads in the archives. Any help will be appreciated.
Thanks in advance-
Todd
