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Hello Todd, Todd Yeates did something like this with actin. Proc Natl Acad Sci U S A. 2005 September 13; 102(37): 13105–13110. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=16141336 HTH Christian Green, Todd wrote: > > Hello all, > > I am working with two proteins that have a relatively low affinity for > each other. I thought I might have more success crystallizing this > complex if I crosslinked the two proteins prior to crystallization > trials. Can someone point me in the direction of reference material > for methods or examples of proteins that have been crosslinked and > subsequently crystallized? I know this has been asked on the ccp4bb > before however I have not seen responses to such threads in the > archives. Any help will be appreciated. > > Thanks in advance- > Todd >
