Sarathy (and Todd)
DMF is usually used for dimethyl formamide. From the name I have no idea what
"dimethyl fluoride" may be (methylene difluoride, though wrong, is used for
difluor methane,but that's not mixable with water, and only a liquid at low
temperature ;-) )
Todd,
If your protein has a sufficiently high affinity for your small molecule, 15 uM
and solid organic compound should work fine (if you wait long enough). In some
cases, if there is residual solubility of a compound, just adding it as a solid
works.
Jens
Parthasarathy, Gopalakrishnan wrote:
Hi Todd,
DMF (Dimethyl Fluoride) is a good alternative to DMSO.
Sarathy
------------------------------------------------------------------------
*From:* CCP4 bulletin board [mailto:[EMAIL PROTECTED] *On Behalf Of
*Green, Todd
*Sent:* Monday, January 22, 2007 3:40 PM
*To:* [email protected]
*Subject:* crystal friendly solvents that are useful for dissolving
hydrophobic small molecules?
Hello All,
I am trying to soak some crystals with a small molecule that is quite
hydrophobic. I am having trouble with solubilty of the small molecule.
It will dissolve up to about 1 mM in 100 % DMSO, but precipitates at
concentrations of less than 15 micromolar when the DMSO concentration is
below 20 percent in my crystal growth solutions(which are peg 4k, low
pH, low salt). Can anyone suggest solvents other than DMSO which might
help dissolve the inhibitor and might be somewhat friendly to my crystals.
Thanks in advance-
Todd Green
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