CCP4 bulletin board <[email protected]> wrote on 02/08/2007 06:34:44 AM:
> Thanks for the links to the boards! > > But I think you could also answer my question. I need a way to remove > gas, solved in my protein sample after using pressure filtration. My > problem is the small sample volume of 400 mikroliters. > > > greets Justin > > Hi Justin - I was also going to suggest the MicroCal degasser; of course you have to have the equipment already. Some other, lower-tech possibilities: - Put the sample in a 15-ml Falcon tube. Attach house vacuum to the top of the tube - they can usually take it without cracking. Tap tube vigorously on the benchtop to nucleate the bubbles. - A brief spin in a speedvac might work - just don't leave it in or you'll dry the sample out. - Don't use pressure filtration. Try a small-volume centrifugal filter instead. Like these: http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=82031-362&inE=1&highlight=82031-362 Disclaimer: I haven't tried the first two methods for this purpose. The first method is similar to how I degassed my calorimetry samples before Microcal introduced its degasser. You might want to try it on a dry tube first... - Matt -- Matthew Franklin , Ph.D. Senior Scientist, ImClone Systems 180 Varick Street, 6th floor New York, NY 10014 phone:(917)606-4116 fax:(212)645-2054 Confidentiality Note: This e-mail, and any attachment to it, contains privileged and confidential information intended only for the use of the individual(s) or entity named on the e-mail. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that reading it is strictly prohibited. If you have received this e-mail in error, please immediately return it to the sender and delete it from your system. Thank you.
