Put it in 1 ml syringe, seal the end with your finger (use a piece of
parafilm in between if you need it) and then draw out the plunger to
create negative pressure, and your bubbles will go away. Then let it
gravity-drain or very slowly push the contents into an eppindorf tube.
or
Speed-vac it for about 1 or 2 minutes (not long enough to lose volume).
On Thu, 8 Feb 2007, Justin Schmitz wrote:
Thanks for the links to the boards!
But I think you could also answer my question. I need a way to remove gas,
solved in my protein sample after using pressure filtration. My problem is
the small sample volume of 400 mikroliters.
greets Justin
P.s. special thanks to James for his constructive advice!!
