Hi all. I am trying to solve a structure with one molecules in the asu through MR. Using a not good data set at 3.0A and a structure with sequence similarity of 37%, Phaser gave a result of z score 14.7 and LL-gain 220, probably a right solution. But when I slightly modified the model I got from Phaser and used it as an input to run Phaser again, I got another solution overlapped partly with the first solution. I don't know how it could happen like that. And the Rfree factor could not go below 0.43 in the following refinemet. I am wondering what is wrong with it? thanx in advance Lei Yin
Lei Yin, Ph.D. National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences No. 15 Datun Road, Beijing 100101, China E-mail:[EMAIL PROTECTED] Tel: 86-10-64888548
