Hi, A non-ccp4 Q. Sorry.
I would like to use a cleavable purification tag at the N-terminus/ extracellular end of my membrane protein for purification. Before I start, I wonder if someone could recommend a particular protease site that I can engineer between the tag and my protein? How about a proprietary cleavage system such as the PreScission protease (GE Healthcare)? I would be grateful to hear success and horror stories in this area.
Best wishes, Rene ================================================ Dr R.A.W. Frank, PhD Royal Commission for the Exhibition of 1851 Research Fellow Prof Seth Grant Lab / Genes to Cognition Wellcome Trust Sanger Institute Hinxton Cambridge CB10 1SA Work Tel: 0044 (0)1223 834244 ext. 7318 Cell No.: 0044 (0)7870 208280 ===============================================
