We have tried them and had them work for a few proteins that didn't
look hopeful other ways.
The chaperones express well and obviously by gel and, in fact, we had
one protein that was difficult to separate from the Cpn60.
We very carefully follow the instructions on induction that come with
the cells (they are a little odd) and do the expression at 10°C.
We have added the ArcticExpress into our standard list of things to
try for rescuing insoluble proteins. We just started testing it out
a few weeks ago and it hasn't saved all of problem proteins by any
means. I don't have the numbers right now, but I think we got
something like 3 of 10 that looked insoluble at 15°C in BL21.
Best of luck,
Cynthia
On Mar 24, 2007, at 9:51 AM, Hongmin Li wrote:
Hi Weijun and all,
Anyone tried the Stratagene's ArcticExpress™ Competent Cells for
expression of proteins at low temperature (13 degree). The company
claims that the cells encode chaperonins Cpn60 and Cpn10 and they
can enhance the protein folding and solubility.
Hongmin
>Dear All,
>I got enclusion body in many cases when I tried to express human
>proteins in E coli. I would like some suggestions on how to go about
>it. I would also like to try co-expression of GroEL/GroES or
>DnaJ/DnaK, and would like to know where to get the plasmids.
>Any help or comments would be appreciated.
>Weijun
____________________
Cynthia Kinsland, Ph.D.
Cornell University
Protein Facility Director
607-255-8844
