Hi Neeraj, There are several ways to approach this problem. Of course, this also depends on your sample and the amount you have available.
1. Probably the first thing to try is seeding (streak or micro). 2. You could try with higher protein and/or precipitant concentration. 3. If you have enough material you could try out what happens at different crystallization temperatures but this is difficult to predict. 4. Additives can make a difference and you could screen using one of the commercially available kits. 5. A more laborious approach would be to change the construct/sample (e.g. removing floppy parts, using binding partners/ligands, optimizing binding partners). Good luck, christian Neeraj wrote: > hi all, > I am working on a protein for which we get nicely diffracting > crystals but the problem is that the crystals grow from anywhere between > 4-6 months. Does anyone has any general suggestions as to what things > can be changed or tried to speed up the process. Any help or suggestions > would be welcome as i am crawling towards getting enough crystals to > make a synchroton visit:):) > > thanks > Neeraj > _______________________________________________________________________ Dr. Christian Biertümpfel Laboratory of Molecular Biology NIDDK/National Institutes of Health phone: +1 301 402 4647 9000 Rockville Pike, Bldg. 5, Rm. B1-03 fax: +1 301 496 0201 Bethesda, MD 20892-0580 USA _______________________________________________________________________
