Hi Neeraj, Two more tricks: 1. Open the cover slip a bit to allow for faster evaporation. It worked well on one of my proteins. 2. in-situ proteolysis: Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt 10):1041-5.
Good luck. Zhen Quoting Neeraj <[EMAIL PROTECTED]>: > hi all, > I am working on a protein for which we get nicely > diffracting > crystals but the problem is that the crystals grow from anywhere > between > 4-6 months. Does anyone has any general suggestions as to what > things > can be changed or tried to speed up the process. Any help or > suggestions > would be welcome as i am crawling towards getting enough crystals > to > make a synchroton visit:):) > > thanks > Neeraj > > -- > Neeraj Kapoor > TPCB Graduate Fellow > Sakmar Lab/ Molecular Biology & Biochemistry > The Rockefeller University > 1230 York Avenue, RRB 510 > New York, NY 10021 > lab.1.212.327.8284:fax.7904 > mobile: 917.535.2030 > http://www.rockefeller.edu/labheads/sakmar/sakmar-lab.html > -- Zhen Zhang Postdoctoral Associate Howard Hughes Medical Institute Department of Biochemistry and Molecular Biophysics Columbia University
