Thank you all.
I think i know the difference.
This is quite a interesting case.


On 8/10/07, Paul Emsley <[EMAIL PROTECTED]> wrote:
>
>
>
> If I may expand on what Eleanor said.
>
> The issue is not the atom labels, but that the atom labels are in the
> right place (i.e. the refer to the right atoms).
>
> There is an IUPAC convention on the torsion of CB-CG-CD-OE1 (for GLU).
> The torsion should be between -90 and +90.  Yours is not.
>
> Therefore the atom labels for OE1 and OE2 should be swapped.
>
> Many molecular graphics or model manipulation programs can fix this.
> In Coot, one would use the fix-nomenclature-errors function.
>
> Hope that is clearer,
>
> Paul.
>
> On Fri, Aug 10, 2007 at 07:19:44PM +0800, Jiamu Du wrote:
> > Dear Eleanor:
> > But in my structure, the atom labelles are all right.
> > Such as GLU A  15  :
> >
> > ATOM     55  N   GLU A  15     -59.930 -39.249  -9.123  1.00 30.10
> > N
> > ATOM     56  CA  GLU A  15     -60.573 -38.776  -7.889  1.00 30.54
> > C
> > ATOM     57  CB  GLU A  15     -61.533 -37.599  -8.146  1.00 31.08
> > C
> > ATOM     58  CG  GLU A  15     -61.014 -36.528  -9.124  1.00 35.70
> > C
> > ATOM     59  CD  GLU A  15     -61.459 -36.784 -10.589  1.00 40.21
> > C
> > ATOM     60  OE1 GLU A  15     -60.561 -36.946 -11.467  1.00 40.16
> > O
> > ATOM     61  OE2 GLU A  15     -62.701 -36.815 -10.851  1.00 41.17
> > O
> > ATOM     62  C   GLU A  15     -61.334 -39.901  -7.182  1.00 29.34
> > C
> > ATOM     63  O   GLU A  15     -61.335 -39.989  -5.947  1.00 29.37
> > O
> >
> > I think the labbels are all right.
> >
> > Thanks.
> >
> > On 8/10/07, Eleanor Dodson <[EMAIL PROTECTED]> wrote:
> > >
> > > There are conventions on which atoms is labelled OE1 and which OE2 for
> > > example
> > >
> > > It doesnt change the electron density of structure calns an iouta but
> > > conventions are conventions!
> > >
> > > Eleanor
> > >
> > > Jiamu Du wrote:
> > > > Dear All:
> > > > I have refined a structure. When I run procheck, a messege apears in
> > > > the log file as below:
> > > >
> > > > Average value of CA-N-C-CB angle is  33.83
> > > >  Standard deviation is  1.85
> > > >  * Side chain atoms swapped for residues:
> > > >  * GLU A  15  ARG A  24  TYR A
> > > >
> > > > I have checked the density and the geometry of these residues. All
> > > > this residues are all right.
> > > > Is there anyone who have the same experience? How to deal with this
> > > > situation?
> > > >
> > > > Thanks.
> > > >
> > > > --
> > > > Jiamu Du
> > > > State Key Laboratory of Molecular Biology
> > > > Institute of Biochemistry and Cell Biology Shanghai Institutes for
> > > > Biological Sciences
> > > > Chinese Academy of Sciences (CAS)
> > >
> > >
> >
> >
> > --
> > Jiamu Du
> > State Key Laboratory of Molecular Biology
> > Institute of Biochemistry and Cell Biology Shanghai Institutes for
> > Biological Sciences
> > Chinese Academy of Sciences (CAS)
>



-- 
Jiamu Du
State Key Laboratory of Molecular Biology
Institute of Biochemistry and Cell Biology Shanghai Institutes for
Biological Sciences
Chinese Academy of Sciences (CAS)

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