The precipitate does not disappear automaticly if I don't add DTT. If it's the precipitate of imidazole, then why DTT can dissolve it? thanks
On 11/13/07, Sanishvili, Ruslan <[EMAIL PROTECTED]> wrote: > This may not apply in your case but it is not uncommon for a protein to > "precipitate" in a microcrystalline shower when put in cold. Once it > worms up, the crystals dissolve and the precipitate clears up. It is > easy to check under a high magnification microscope. > Cheers, > N. > > > Ruslan Sanishvili (Nukri), Ph.D. > > GM/CA-CAT, Bld. 436, D007 > Biosciences Division, ANL > 9700 S. Cass Ave. > Argonne, IL 60439 > > Tel: (630)252-0665 > Fax: (630)252-0667 > [EMAIL PROTECTED] > > > > > -----Original Message----- > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of > Joe > Sent: Tuesday, November 13, 2007 2:30 PM > To: [email protected] > Subject: [ccp4bb] DTT sensitive? > > Hi there, > I see enormous precipitate of my receptor protein when I take it out of > freezer. But all the precipitate dissolved quickly after I added 1mM DTT > to the solution. Does this mean that some surface Cys are causing > problem? Or why is the protein so sensitive to DTT? > Anybody experienced this kind? > Any advice is appreciated. > -Joe >
