It did not contain DTT when frozen.
On 11/13/07, deena <[EMAIL PROTECTED]> wrote: > Are you sure its 1mM DTT, because DTT itself precipitates in the freezer. > > Deena > > > On Nov 13, 2007, at 3:30 PM, Joe wrote: > > Hi there, > I see enormous precipitate of my receptor protein when I take it out > of freezer. But all the precipitate dissolved quickly after I added > 1mM DTT to the solution. Does this mean that some surface Cys are > causing problem? Or why is the protein so sensitive to DTT? > Anybody experienced this kind? > Any advice is appreciated. > -Joe > > Deena Abells Oren, PhD > Manager, Structural Biology Resource Center > Rockefeller University > 1230 York Ave > New York, NY 10065-6399 > phone: 212-327-7429 > >
