If you have a fluorescence microscope, you could try soaking SYBR-Gold dye into your crystals.
Kettenberger, H., and Cramer, P. (2006). Fluorescence detection of nucleic acids and proteins in multi-component crystals. Acta Crystallogr D Biol Crystallogr 62, 146-150. Alan Rongjin Guan wrote: > Dear All > > I am trying to co-crystallize a protein and dsRNA complex, and looking > for methods > to detect RNA in the crystals. I am thinking about using Mass Spectrum, > Nanodrop to > measure OD280/260, etc, but I wonder if ther are some more sensitive and > reliable ways. > > The crystals are grown in high concentration salts; the size is usually > small but I do > have several big ones which can be easily fished out. They can be dyed > so definitely > not salt crystals. > > Thanks > > Rongjin Guan -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E-mail: [EMAIL PROTECTED]
