You could stain your crystals directly with Sybr Gold, which will fluoresce upon binding nucleic acids, or you can visualize both protein and RNA on a silver-stained SDS-PAGE gel.
Kevin Rongjin Guan wrote: > Dear All > > I am trying to co-crystallize a protein and dsRNA complex, and > looking for methods to detect RNA in the crystals. I am thinking > about using Mass Spectrum, Nanodrop to measure OD280/260, etc, but I > wonder if ther are some more sensitive and reliable ways. > > The crystals are grown in high concentration salts; the size is > usually small but I do have several big ones which can be easily > fished out. They can be dyed so definitely not salt crystals. > > Thanks > > Rongjin Guan
