Hi Jeroen,
We used a BioRad iCycler iQ for the measurements in
"Thermofluor-based high-throughput stability optimization of proteins
for
structural studies." Ericsson, et. al. Analytical Biochemistry (2006)
357:289-298.
The wavelengths for excitation and detection were ~490 and ~575 nm,
respectively (for Sypro Orange).
See http://probes.invitrogen.com/handbook/figures/0020.html for
wavelength (band) selection.
The iCycler iQ is highly dependable and very sensitive.
Best regards,
Martin
On Feb 12, 2008, at 6:07 PM, mesters wrote:
Sorry for the off-topic but can somebody recommend highly a
sensitive RT-PCR machine for the thermofluor experiment (sypro
orange).
That would imply excitation below 500 nm (ideally 470) and detection
at about 570 nm, right? I know several simple machines have a
problem with the 570 nm...
Jeroen.
.
B. Martin Hallberg, PhD
Assistant professor
Department of Cell and Molecular Biology
Medical Nobel Institute
Karolinska Institutet
von Eulers v. 1
SE-171 77 Stockholm
Sweden
