Hei Jeroen,
Quite recently we got BioRad MiniOpticon system for the thermoflor based
assays. I think this is the least expensive RT-PCR on the market but it performs
very well in our experiments.
It can excite the samples between 470 and 505 nm and one of the detection
channel covers the Sypro Orange emission.
The control software is VERY easy to use.
Andrzej, HY
mesters wrote:
Sorry for the off-topic but can somebody recommend highly a sensitive
RT-PCR machine for the thermofluor experiment (sypro orange).
That would imply excitation below 500 nm (ideally 470) and detection at
about 570 nm, right? I know several simple machines have a problem with
the 570 nm...
Jeroen.
