Hi Jeroen, I assume you are planning to use Sypro Orange. We are using the Roche LC480 (simply because it's around in the lab next door) and it does the job although it's a little inconvenient to tweak the settings.
Many people use the iCycler from Biorad. This machine is a little more flexible and can be customized: You can introduce filter pairs of your choice and play with other dyes such as ANS-1.8. We intend to do that since sypro orange binds to quite a number of our proteins before heating (which perhaps tells you something about our proteins but lets blame the dye first). Ralf Ralf Jauch Genome Institute of Singapore 60 Biopolis Street #02-01Genome Singapore 138672 Tel: (65) 6478 8102 Mobile: (65) 96398715 [EMAIL PROTECTED] -----Ursprüngliche Nachricht----- Von: CCP4 bulletin board im Auftrag von mesters Gesendet: Mi 13.02.2008 01:07 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] Thermofluor Sorry for the off-topic but can somebody recommend highly a sensitive RT-PCR machine for the thermofluor experiment (sypro orange). That would imply excitation below 500 nm (ideally 470) and detection at about 570 nm, right? I know several simple machines have a problem with the 570 nm... Jeroen. -- Jeroen Raymundus Mesters, Ph.D. Institut fuer Biochemie, Universitaet zu Luebeck Zentrum fuer Medizinische Struktur und Zellbiologie Ratzeburger Allee 160, D-23538 Luebeck Tel: +49-451-5004070, Fax: +49-451-5004068 E-mail: [EMAIL PROTECTED] Http://www.biochem.uni-luebeck.de Http://www.iobcr.org Http://www.opticryst.org -- If you can look into the seeds of time and say which grain will grow and which will not - speak then to me (Macbeth) --