Hi, You can use dimethylamine borane (DMAB) for reductive methylation of the surface exposed K's. You may also try NaBH4 or sodium cyanoborohydride but the first may be best. Don't know about D's and E's.
Best, Debanu. -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Jacob Keller Sent: Thursday, February 21, 2008 2:32 PM To: [email protected] Subject: [ccp4bb] Side chain chemical modifiers Dear Crystallographers, I am looking for a way to modify any specific set of sidechains in my protein (e.g., all K's, or all D's and E's, etc.). A few requirements: 1. Relatively cheap 2. Definitely non-cell-membrane-crossing 3. Specific 4. Any reasonable amount of change in mass (+ 5-5000 Da?), charge, etc., is fine 5. Preferably less life threatening to live cells (I know this is a probably a losing battle) The last criterion is because I want to modify the protein in vivo, then immediately harvest the cells, etc. I was hoping there would be something "around the house" that I could use for this, especially for hitting K's. All the best, Jacob ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: [EMAIL PROTECTED] *******************************************
