Oh...... Thank you. I will try to fit it with a piece of PEG. Best wishes. On Tue, Feb 26, 2008 at 10:38 PM, <[EMAIL PROTECTED]> wrote:
> Hi Jiamu, > > This is almost surely a nic piece you PEG3350. > > Regards, > > zsolt > > > Dr. Zsolt Bocskei > sanofi-aventis > Chemical Sciences Department > 16 rue d'Ankara > 67000 Strasbourg > France > > ------------------------------ > *From:* CCP4 bulletin board [mailto:[EMAIL PROTECTED] *On Behalf Of *Jiamu > Du > *Sent:* Tuesday, February 26, 2008 3:33 PM > *To:* [email protected] > *Subject:* [ccp4bb] unknown symmetric density > > Dear All, > Here I am refining a 2.0 angstrom resolution structure of a protein in > complex with a 18mer peptide. Now the refined model has a R/Rfree value of > 0.206/0.266. But there is no obvious density for the peptide. An > interesting observation is that some strange density is located in the > proposed protein-peptide interaction interface as shown in the attachment. > The shown map is SACO map at 1.0 sigma level. The strange density is near > the 2-fold axis of the crystal cell, so the density is also 2-fold > symmetric. Although I traced it with solvent molecules, I believe it is not. > It seems not the peptide, either. But I can not image what it is. What do > you think? > The crystal condition contains Tris, Hepes, NaCl, ZnAc, and PEG3350. > Thanks. > > -- > Jiamu Du > State Key Laboratory of Molecular Biology > Institute of Biochemistry and Cell Biology Shanghai Institutes for > Biological Sciences > Chinese Academy of Sciences (CAS) > -- Jiamu Du State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences (CAS)
