Hmm....somehow this seems to sit on a twofold as well...see 2.jpg Will be interesting to model..
BR -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Mario Sanches Sent: Tuesday, February 26, 2008 11:40 AM To: [email protected] Subject: Re: [ccp4bb] unknown symmetric density Same thing to me. I was able to assign a PEG molecule to a similar density. Mario Sanches Jiamu Du wrote: > Oh...... > Thank you. I will try to fit it with a piece of PEG. > Best wishes. > > On Tue, Feb 26, 2008 at 10:38 PM, <[EMAIL PROTECTED] > <mailto:[EMAIL PROTECTED]>> wrote: > > Hi Jiamu, > > This is almost surely a nic piece you PEG3350. > > Regards, > > zsolt > > > Dr. Zsolt Bocskei > sanofi-aventis > Chemical Sciences Department > 16 rue d'Ankara > 67000 Strasbourg > France > > > ------------------------------------------------------------------------ > *From:* CCP4 bulletin board [mailto:[email protected] > <mailto:[email protected]>] *On Behalf Of *Jiamu Du > *Sent:* Tuesday, February 26, 2008 3:33 PM > *To:* [email protected] <mailto:[email protected]> > *Subject:* [ccp4bb] unknown symmetric density > > Dear All, > Here I am refining a 2.0 angstrom resolution structure of a > protein in complex with a 18mer peptide. Now the refined model has > a R/Rfree value of 0.206/0.266. But there is no obvious density > for the peptide. An interesting observation is that some strange > density is located in the proposed protein-peptide interaction > interface as shown in the attachment. The shown map is SACO map at > 1.0 sigma level. The strange density is near the 2-fold axis of > the crystal cell, so the density is also 2-fold symmetric. > Although I traced it with solvent molecules, I believe it is not. > It seems not the peptide, either. But I can not image what it is. > What do you think? > The crystal condition contains Tris, Hepes, NaCl, ZnAc, and PEG3350. > Thanks. > > -- > Jiamu Du > State Key Laboratory of Molecular Biology > Institute of Biochemistry and Cell Biology Shanghai Institutes for > Biological Sciences > Chinese Academy of Sciences (CAS) > > > > > -- > Jiamu Du > State Key Laboratory of Molecular Biology Institute of Biochemistry > and Cell Biology Shanghai Institutes for Biological Sciences Chinese > Academy of Sciences (CAS)
