Same thing to me. I was able to assign a PEG molecule to a similar density.
Mario Sanches
Jiamu Du wrote:
Oh......
Thank you. I will try to fit it with a piece of PEG.
Best wishes.
On Tue, Feb 26, 2008 at 10:38 PM, <[EMAIL PROTECTED]
<mailto:[EMAIL PROTECTED]>> wrote:
Hi Jiamu,
This is almost surely a nic piece you PEG3350.
Regards,
zsolt
Dr. Zsolt Bocskei
sanofi-aventis
Chemical Sciences Department
16 rue d'Ankara
67000 Strasbourg
France
------------------------------------------------------------------------
*From:* CCP4 bulletin board [mailto:[email protected]
<mailto:[email protected]>] *On Behalf Of *Jiamu Du
*Sent:* Tuesday, February 26, 2008 3:33 PM
*To:* [email protected] <mailto:[email protected]>
*Subject:* [ccp4bb] unknown symmetric density
Dear All,
Here I am refining a 2.0 angstrom resolution structure of a
protein in complex with a 18mer peptide. Now the refined model has
a R/Rfree value of 0.206/0.266. But there is no obvious density
for the peptide. An interesting observation is that some strange
density is located in the proposed protein-peptide interaction
interface as shown in the attachment. The shown map is SACO map at
1.0 sigma level. The strange density is near the 2-fold axis of
the crystal cell, so the density is also 2-fold symmetric.
Although I traced it with solvent molecules, I believe it is not.
It seems not the peptide, either. But I can not image what it is.
What do you think?
The crystal condition contains Tris, Hepes, NaCl, ZnAc, and PEG3350.
Thanks.
--
Jiamu Du
State Key Laboratory of Molecular Biology
Institute of Biochemistry and Cell Biology Shanghai Institutes for
Biological Sciences
Chinese Academy of Sciences (CAS)
--
Jiamu Du
State Key Laboratory of Molecular Biology
Institute of Biochemistry and Cell Biology Shanghai Institutes for
Biological Sciences
Chinese Academy of Sciences (CAS)
