On Mar 12, 2008, at 12:48 PM, Jan Schoepe wrote:
Hello everybody,
I wonder if anybody has experience with heme (or to be more precise:
heme b) containing proteins which Xtals do not look red under the
microscope. How might the technique for crystallization (e.g.
sitting drop, hanging drop) influence the intensity of the color?
Many thanks!
If there is no metal in the heme, its color will be very different.
If your crystallization buffer contains EDTA or other strong chelating
agents, I imagine this might happen, though protein crystallization is
not my area.
Ian
