Re: [ccp4bb] Color of heme containing Xtals

[Dominique BOURGEOIS]

Hello, A more precise view on the spectroscopic signature of your crystalline heme protein can be obtained by microspectrophotometry (UV-vis absorption, Raman or fluorescence). For such studies, people are welcome to apply for using the Cryobench lab at  ESRF  (http://www.esrf.eu/UsersAndScience/Experiments/MX/Cryobench/). A number of microspec are also lying around or can be purchased (for example: http://www.4dx.se/spectrop.htm). Dominique. Jan Schoepe a écrit : Hello everybody, I wonder if anybody has experience with heme (or to be more precise: heme b) containing proteins which Xtals do not look red under the microscope. How might the technique for crystallization (e.g. sitting drop, hanging drop) influence the intensity of the color? Many thanks! Jan Lesen Sie Ihre E-Mails jetzt einfach von unterwegs.. -- Dominique BOURGEOIS /\ Dominique Bourgeois /\/\ / \ LCCP IBS /\/ /\ /\/ \ UMR 5075 / \/ \ / \ \ 41 Rue Jules Horowitz \ \ \ \ 38027 GRENOBLE Cedex 1 \ \ \ \ FRANCE \ \ ****** phone: (+33) (0)4 38 78 96 44 ****** ****** fax: (+33) (0)4 38 78 51 22 ****** ****** e-mail: [EMAIL PROTECTED] ***