There are quite a number of structures homodimers and homotetramers in the PDB where the dissociation constant is known to be in the millimolar range. For example the dimerizaion of a humainized antibody VHH domain that mimicks a VH-VL complex (Conrath et al. J. Mol. Biol. (2005) 350, 112125).
Remy Loris Vriej universiteit Brussel >Hi, > >Can anyone advise me what is currently the weakest protein-protein >complex yet crystallised? Google searching turned up a paper from the >Tromsø crystallography group (Helland et al. 1999, JMB 287, 923942) >in which a complex between beta-trypsin and a P1 mutant of BPTI with a >Kd of 68 uM was described as belonging to the weakest complexes solved >to date, but this article was from 1999 and much water has passed >under the bridge since then. > >Thanks >Derek >_________________________________________________________________ >Derek Logan tel: +46 46 222 1443 >Associate professor fax: +46 46 222 4692 >Molecular Biophysics mob: +46 76 8585 707 >Centre for Molecular Protein Science >Lund University, Box 124, 221 00 Lund, Sweden > > > > > > > >
