You could try the matrix seeding method, popularised by Allan D'Arcy et al (Acta Cryst. (2007). D63, 550-554)
Crush up your needles, and use them to seed into a new round of screening. You may be able to jump into a new crystal system this way Janet -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Marta MartÃnez Sent: Tuesday, 1 July 2008 5:03 PM To: [email protected] Subject: [ccp4bb] needles not improved Hi Anyone can help me?. I have crystals that are needles (with twinning, apparently). The crystalization condition is PEG 4K, 0.1 M NaAc pH 4.6 and 0.2 M ammonium acetate. I have tried in this condition with detergents and additives and with ligands but nothing improves the needles. Always needles. Are there any other stategies to try?. Thank you Marta e-mail: [EMAIL PROTECTED] >
