Dear All, I want to prepare 25 mM sodium acetate buffer pH 4.5. can anyone give the exact composition of how to prepare it. we prepare it using sodium acetate and acetic acid combination. i am not able to arrive at the calculatation correctly, so if anyone can explain me with the above buffer how to calculate. and what sodium acetate [Anhydrous / trihydrate] and acetic acid [glacial/ plain] to use.
thanks n regards Meg goyal, M.SC Biotechnology [Research] Institute of science, Fort Mumbai, INDIA
