So what, then, will be the concentration of the acetate ion in your
stock solution when you have finished?
(Disclaimer: I get to teach this stuff periodically in remedial
chemistry as a punishment for deployment of excessive sarcasm during
faculty meetings.)
On Jul 22, 2008, at 6:10 AM, Santosh wrote:
Hi,
Make a 1M Na-Acetate do not make up to the 1 Ltr volume. Leave some
extra
volume and now start adding Acetic acid till you get pH 4.5 (Glacial
Acetic
Acid).
Now make up the volume to 1ltr or how much ever you are deciding to
make the
50X stock solution.
Best,
Santosh
On Mon, Jul 21, 2008 at 11:20 PM, William G. Scott <
[EMAIL PROTECTED]> wrote:
This is a job for the trusty Henderson-Hasselbalch equation:
http://en.wikipedia.org/wiki/Henderson-Hasselbalch_equation
On Jul 21, 2008, at 8:12 PM, Meg wrote:
Dear All,
I want to prepare 25 mM sodium acetate buffer pH 4.5. can anyone
give the
exact composition of how to prepare it. we prepare it using sodium
acetate
and acetic acid combination. i am not able to arrive at the
calculatation
correctly, so if anyone can explain me with the above buffer how to
calculate. and what sodium acetate [Anhydrous / trihydrate] and
acetic
acid
[glacial/ plain] to use.
thanks n regards
Meg goyal,
M.SC Biotechnology [Research]
Institute of science,
Fort
Mumbai, INDIA
