I recently had a case, unpublished right now, where the NMR structure
of the monomer was determined and all other biochemical evidence
showed a monomer as the active form. The resulting crystal structure
turned out to be a domain swapped dimer. The group I did the work for
are still puzzling over it and have tried a number of things to see if
the structure is biologically significant.
Leonard Thomas Ph. D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
620 Parrington Oval
Norman, OK 73032
[email protected]
Office: 405-325-1126
Lab: 405-325-7571
On Dec 11, 2008, at 10:09 AM, Santarsiero, Bernard D. wrote:
In parallel with the discussion around this off-CCP4-topic, are they
any
good examples of the opposite case, where the protein is a monomer in
solution (as evident from light scattering, MW determination through
centrifugation, EPR, etc.) but crystallizes as a dimer or higher
multimer?
Bernie Santarsiero
