Hi,
My crystal was grown in 33%MPD/0.2M (NH4)2SO4/5% P400/0.1M tris in 500 ul well covered with 200ul paraffin oil. The droplet (0.9 ul protein + 0.9 ul reservoir + 0.2 ul 30% DMSO) is hung over the cover slide. Because of twining issue, when the small but single crystal showed up from the drop after overnight setting up, I transferred the cover slide to 30% MPD condition well IMMEDIATELY. (When crystal gets older, it tends to grow into multilayer form, stacking plate lies on the first crystal) HOWEVER, after inspecting under microscope, I found that there were tremendous tiny crystals surround those single crystals :( Any suggestion to improve this situation in order to avoid introducing any tiny crystal in the drop. Additional Q: Should I include 3% DMSO in my cryosolution? Regards, HengChiat _________________________________________________________________ Windows Live™ SkyDrive™: Get 25 GB of free online storage. http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_SD_25GB_062009
