Have you tried acorn in ccp4? I've had it work well at this resolution,
especially if the protein/peptide has some alpha helical content. We used
acorn to solve a small cro protein that we couldn't get molecular replacement
to work with by using a 5-residue ideal poly-ala helix as the starting model.
Sue
Quoting "Sickmier, Allen" <[email protected]>:
I am trying to do molecular replacement on a small peptide (less than
40 AA) and have not had any success using phaser. Are there any
tricks or better programs for really small peptides?
The data is great 1.1 A, ~35% solvent, and two molecules in the ASU.
I have tried all the standard stuff, changing resolution cut off etc.
I may move to sulfur phasing at this resolution but I would like to
get the MR to work.
Allen
