In short, yes you can. Although it is often avoided due to the limitations that high salt samples may have, you still have a good chance to crystallize the protein in the original high salt buffer. In my case a small alcohol was the best precipitating agent. As with most proteins, try it, try it, try it. First find the correct buffer condition where your protein is stable and monomodal (DLS is a good tool to check this), and then start screening.
Good luck, Djordje > Dear All, > I have a peri domain protein that is stable in high salt concentration(500 > mM), if I dialysis to a lower salt buffer and then concentrate, it'll > preticipate out. If I need to crystallize it, can I use the high salt > buffer? Is there any optimization kits that could help to increase the > solubility? Thanks. >
