> -----Original Message----- > From: [email protected] [mailto:[email protected]] On > Behalf Of Francis E Reyes > Sent: 21 July 2009 22:22 > To: [email protected] > Subject: Self Rotation map in R32? > > Hi all > > I'm trying to understand why there is a peak that is perpendicular to > the c axis on my kappa = 180 and kappa = 90?
On the kappa = 180 section they are the 3 crystallographic 2-folds perp to the cryst 3-fold parallel to c. On kappa = 90 a peak would be a 4-fold but since there's no obviously no crystallographic 4-fold in R32, that would imply 4-fold NCS, which would be unusual. You didn't post your kappa=90 section so we can't tell if it really is a significant peak. > The chapter entitled "Characterizing a Crystal From an Initial Native > Dataset" from Sawaya says that R32 shouldn't have a central peak in > kappa = 180 nor 90. Not sure what you mean by 'central peak', the peak in the middle of the kappa=180 section is the crystallographic 3-fold so that's unavoidable in R32. Again any peak on kappa=90 would depend on what kind of NCS you have. How many mols/a.u. do you estimate? HTH! -- Ian Disclaimer This communication is confidential and may contain privileged information intended solely for the named addressee(s). It may not be used or disclosed except for the purpose for which it has been sent. If you are not the intended recipient you must not review, use, disclose, copy, distribute or take any action in reliance upon it. If you have received this communication in error, please notify Astex Therapeutics Ltd by emailing [email protected] and destroy all copies of the message and any attached documents. Astex Therapeutics Ltd monitors, controls and protects all its messaging traffic in compliance with its corporate email policy. The Company accepts no liability or responsibility for any onward transmission or use of emails and attachments having left the Astex Therapeutics domain. Unless expressly stated, opinions in this message are those of the individual sender and not of Astex Therapeutics Ltd. The recipient should check this email and any attachments for the presence of computer viruses. Astex Therapeutics Ltd accepts no liability for damage caused by any virus transmitted by this email. E-mail is susceptible to data corruption, interception, unauthorized amendment, and tampering, Astex Therapeutics Ltd only send and receive e-mails on the basis that the Company is not liable for any such alteration or any consequences thereof. Astex Therapeutics Ltd., Registered in England at 436 Cambridge Science Park, Cambridge CB4 0QA under number 3751674
