No there are 6 rotational equivalent positions in R32 (i.e. not counting the translational R centred positions which have no effect on the RF), consisting of 2 groups of 3, call them 1,2,3 and 4,5,6. Members of each group of 3 are internally related by the 3-fold and members of different groups are related by the 2-folds. Each 2-fold peak in the SRF relates 3 pairs of a.u.s (e.g. 1-4, 2-5, 3-6) so will have multiplicity=3. Each 3-fold peak relates 6 pairs: 1-2, 2-3, 3-1, 4-5, 5-6, 6-4 so has multiplicity=6, so should be twice the peak height.
Cheers -- Ian > -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of Francis E Reyes > Sent: 22 July 2009 13:51 > To: Ian Tickle; Francis E Reyes > Cc: CCP4 bulletin board > Subject: RE: [ccp4bb] Self Rotation map in R32? > > >This nicely illustrates the danger of using too low > resolution data to > >compute the SRF (I'm referring to an earlier BB discussion on this > >subject, where it was suggested to cut out the high resolution data, > >against, it seems to me, all rationale). You should be using as high > >resolution valid data as possible (it goes without saying that using > >rubbish data at any resolution won't help!). > > > > The resolution is to 3A. > > > >The 3-fold peak will be *very* strong, it's crystallographic > after all: > >seeing it 'leaking' onto adjacent sections and even > stretching as far as > >the kappa=180 section is common, particularly with low > resolution data > >and/or using F's. > > > Wouldn't the 2-folds then also be equally as strong? > > Thanks for your thoughtful responses. > > FR > > Disclaimer This communication is confidential and may contain privileged information intended solely for the named addressee(s). It may not be used or disclosed except for the purpose for which it has been sent. If you are not the intended recipient you must not review, use, disclose, copy, distribute or take any action in reliance upon it. If you have received this communication in error, please notify Astex Therapeutics Ltd by emailing [email protected] and destroy all copies of the message and any attached documents. Astex Therapeutics Ltd monitors, controls and protects all its messaging traffic in compliance with its corporate email policy. The Company accepts no liability or responsibility for any onward transmission or use of emails and attachments having left the Astex Therapeutics domain. Unless expressly stated, opinions in this message are those of the individual sender and not of Astex Therapeutics Ltd. The recipient should check this email and any attachments for the presence of computer viruses. Astex Therapeutics Ltd accepts no liability for damage caused by any virus transmitted by this email. E-mail is susceptible to data corruption, interception, unauthorized amendment, and tampering, Astex Therapeutics Ltd only send and receive e-mails on the basis that the Company is not liable for any such alteration or any consequences thereof. Astex Therapeutics Ltd., Registered in England at 436 Cambridge Science Park, Cambridge CB4 0QA under number 3751674
