It will help more if we send it to James ;)
On Oct 11, 2009, at 9:15 AM, gauri misra wrote:
Dear James,
As there are indications of protein degradation that have been
suggested in
previous postings, i think adding some protease inhibitors right at
the
stage of purification may provide some help.
Moreover, you can also try various additives as specified in one of
the
Hampton screens (certainly if the protein requires some cofactor for
its
functioning that would be the priority as an additive).
You have just given one condition. Is it that crystals are visible
only in
this particular condition?
If there are positive results in some other condition we can think of
playing around that too as an alternative strategy.
Hope this little piece of suggestion may help you at some point.
Best wishes
Gauri
