Dear James --
One possible issue you might have as well is oxidation. Your sample
might need to be in a different step... and would therefore require
some time before getting to this step. I personally noticed (in few
cases) that when my crystals took time to grow, it was either because
they were degraded, they needed a disulfide bond to be formed
(oxidation level), or they needed to be unfolded / refolded (in a
different conformation), the latest one being a rare case.
HTH.
Kind regards.
-- Leo --
On 12 Oct 2009, at 00:27, james09 pruza wrote:
Dear crystallographers,
Sorry for the non-ccp4 query. I am new to this field and need some
suggestions. My question is, why some protein takes longer time to
crystallize, say 6-8 months, and it is the only condition to get
the crystals.? What are the ways to get the crystals faster.
The crystal appears with 60% of 2-Methyl, 2-4 Pentane Diol and only
at 4 degree with very low concentration of NaCl. I have got some
of the sugggestions earlier from the CCP4-discussion board for
microseeding, but it did not work.
All suggestions from the experts are welcome.
Thanks.
James
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Chavas Leonard, Ph.D.
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