Hi James,
have you tried limited proteolysis on your protein and see if you can
identify a stable fragment. Then re-clone and re-crystallize your
protein. Or a very stupid suggestion, how does your size exclusion
peak look like ? What you're not running your protein over a SEC to
polish it ?
Good luck,
Jürgen
On Oct 11, 2009, at 11:27 AM, james09 pruza wrote:
Dear crystallographers,
Sorry for the non-ccp4 query. I am new to this field and need some
suggestions. My question is, why some protein takes longer time to
crystallize, say 6-8 months, and it is the only condition to get the
crystals.? What are the ways to get the crystals faster.
The crystal appears with 60% of 2-Methyl, 2-4 Pentane Diol and only
at 4 degree with very low concentration of NaCl. I have got some of
the sugggestions earlier from the CCP4-discussion board for
microseeding, but it did not work.
All suggestions from the experts are welcome.
Thanks.
James
-
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Phone: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-3655
http://web.mac.com/bosch_lab/
