Dear Zq Deng, I've had success with the dilution method as described by Dunlop and Hazes: http://scripts.iucr.org/cgi-bin/paper?en5016
For me it worked for a couple of projects, and gives you a bit more to permutate than just varying the protein:mother liquor ratios, as Thomas Edwards helpfully suggested. Good luck, Mark On 6 May 2010 09:03, zq deng <dengzq1...@gmail.com> wrote: > hello,everybody . due to excess nucleation,I often get many tiny crystals > instead of few,large crystals.i wana optimize the condition, does anyone > have adivce about this? > > Best regards. > -- Skype: markabrooks