Dear Zq Deng,
                    I've had success with the dilution method as described
by Dunlop and Hazes: http://scripts.iucr.org/cgi-bin/paper?en5016

For me it worked for a couple of projects, and gives you a bit more to
permutate than just varying the protein:mother liquor ratios, as Thomas
Edwards helpfully suggested.

Good luck,

Mark

On 6 May 2010 09:03, zq deng <dengzq1...@gmail.com> wrote:

> hello,everybody . due to excess nucleation,I often get many tiny crystals
> instead of  few,large crystals.i wana optimize the condition, does anyone
> have adivce about this?
>
> Best regards.
>



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Skype: markabrooks

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