actually,the protein grow in too different condition.both have excess necleation,and one condition is that just puting protein in the drop and not mixing with the precipitants.there is one another problem how to cryo-protect. 2010/5/6 syed ibrahim <b_syed_ibra...@yahoo.com>
> > Hi > > I have succeeded by using oil (such as parafin oil ... etc ) at reservoir > on top of the reservoir solution. You have to try several trials to find > optimum ratio. > > With regards > > Syed > > --- On *Thu, 5/6/10, zq deng <dengzq1...@gmail.com>* wrote: > > > From: zq deng <dengzq1...@gmail.com> > > Subject: [ccp4bb] control of nucleation > To: CCP4BB@JISCMAIL.AC.UK > Date: Thursday, May 6, 2010, 1:33 PM > > > hello,everybody . due to excess nucleation,I often get many tiny crystals > instead of few,large crystals.i wana optimize the condition, does anyone > have adivce about this? > > Best regards. > > >
