Hi Zq Deng
No-one has mentioned microseeding into random screens, the so-called Microseeding Matrix Screening (MMS) method , or just "matrix seeding". This is exactly the kind of situation where the method works really well. You will very often get many new leads, as well as bigger crystals. See the excellent paper by D'Arcy et al., Acta Cryst. (2007). D63, 550-554, and also the original paper of Ireton and Stoddard, Acta Cryst. (2004). D60, 601-605. See also the practical and theoretical comments at http://www.douglas.co.uk/mms.htm Best wishes Patrick -- For information and discussion about protein crystallization and automation, please join our bulletin board at http://groups-beta.google.com/group/oryx_group?hl=en patr...@douglas.co.uk Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of zq deng Sent: 06 May 2010 09:04 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] control of nucleation hello,everybody . due to excess nucleation,I often get many tiny crystals instead of few,large crystals.i wana optimize the condition, does anyone have adivce about this? Best regards.
