Tuhin, I have had some success with low molecular weight PEG eg. PEG200. -bob
On Thu, May 13, 2010 at 1:00 PM, Tuhin Bhowmick <[email protected]> wrote: > Dear All, > does anyone have any clever suggestion/s for handling the solubility problem > of highly hydrophobic compound, during co-crystallization or inhibition > assays? The ligands I am using are almost insoluble in aquous medium > (precipitates out or becomes turbid at ~1 micro molar conc.). In DMSO, the > solubility is upto 50mM. Ligands are not soluble in MeOH or EtOH. > Besides crystallization, this solubility is also a hindrance for > in-vitro or in-vivo assays requiring higher conc. of ligand (keeping in > mind, the final DMSO conc. <5%)! > Some of the ligands have benzoic acid groups. Does it help to convert them > to Na benzoate form? > Thanks for your time, > Tuhin. > > Dept. of Physics > Indian Institute of Science. > > > > > -- > This message has been scanned for viruses and > dangerous content by MailScanner, and is > believed to be clean. >
