As a piece of trivia regarding low-MW-PEG-as-solvent:
the original paper in which the active compound of hashish (THC) was
discovered (Science or Nature 1970's), the active fraction was dissolved in
PEG-300 (I believe that was the MW), and injected into monkeys. If I recall
correctly, the monkeys' appetites increased, their eyes became watery, and
they evinced a "pensive" disposition!
Jacob Keller
ps I always wondered whether this might have actually been due to proteins
crystallizing in their veins...
----- Original Message -----
From: "Robert Immormino" <[email protected]>
To: <[email protected]>
Sent: Thursday, May 13, 2010 4:02 PM
Subject: Re: [ccp4bb] Dissolving Hydrophobic ligands for co-crystallization
and assays
Tuhin,
I have had some success with low molecular weight PEG eg. PEG200.
-bob
On Thu, May 13, 2010 at 1:00 PM, Tuhin Bhowmick
<[email protected]> wrote:
Dear All,
does anyone have any clever suggestion/s for handling the solubility
problem
of highly hydrophobic compound, during co-crystallization or inhibition
assays? The ligands I am using are almost insoluble in aquous medium
(precipitates out or becomes turbid at ~1 micro molar conc.). In DMSO, the
solubility is upto 50mM. Ligands are not soluble in MeOH or EtOH.
Besides crystallization, this solubility is also a hindrance for
in-vitro or in-vivo assays requiring higher conc. of ligand (keeping in
mind, the final DMSO conc. <5%)!
Some of the ligands have benzoic acid groups. Does it help to convert them
to Na benzoate form?
Thanks for your time,
Tuhin.
Dept. of Physics
Indian Institute of Science.
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